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Thromb Res 132:138142

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By leucocyte-depletion of RBC, by inhibiting proteases with phenylmethyl sulfonyl chloride (PMSF), by surface- 125 I -iodinating young and old RBC, by extraction with Triton and addition of a second antibody it was demonstrated that preexisting immune complexes contained band 3 monomers and oligomers in samples from old but not young RBC (Lutz and Stringaro-Wipf, 1983)

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The tissues were counterstained with DAPI (4,6-diamidino-2-phenylindole) for 10 min at room temperature to label the nuclei and then mounted on slides with anti-fade medium (ProLong Gold, Thermo Fisher Scientific Inc., Rockford, IL)