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The supernatants were collected by centrifugation at 5000 rpm for 5 min at 4 C and precleared by incubated with GFP-Trap agarose beads (Chromotek) or HA-Beads (Roche) for 2 h at 4 C with rotation

Biotin has an extremely high affinity toward streptavidin (Kd 1014 nM), enabling cell lysis and affinity capture under harsh conditions and thus the near complete extraction of a cells proteome (Samavarchi-Tehrani et al., 2020)

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Prediction analyses were performed through backward stepwise Cox proportional hazards regression and expressed as the hazard ratio (HR)

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