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Total protein extracts were prepared by standard procedures and analyzed by PAGE using the following antibodies: caspase 3 active form, iNOS (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and Ub (Sigma-Aldrich)

ISBN 978-0-12-182806-6

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Unlike other forms of regulated cell death, cuproptosis is characterized by the direct binding of reduced copper (Cu + ) to lipoylated proteins of the tricarboxylic acid (TCA) cycle, most notably dihydrolipoamide S-acetyltransferase (DLAT), together with destabilization of iron-sulfur (Fe-S) cluster proteins such as succinate dehydrogenase complex ironsulfur subunit B (SDHB) 164

In this situation the material could be injected in the perilimbic cornea interspersed between the corneal lesion and its undesired potential limbic blood supply

Anecdotally, benzyl alcohol also has a mild local anesthetic effect