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Importantly, we demonstrated that pre-activation of the VQ-precursor by a 4-h incubation with methyl vinyl ketone (MVK) enables rapid modification, even when the reaction time is shortened to 10 min (Supplementary Fig

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Thus, the dissociation of Cu(II) ions from HSA in the presence of EDTA is relatively slow and the affinity of Cu(II) for HSA is lower than that for EDTA