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The gradient used started at 2% of buffer B and was increased to 16% over 185 min, and then to 28% over 30 min

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doi: 10.1038/s41418-019-0380-z 47 LiuMKongXYYaoYWangXAYangWWuHet al

IL-24 impairs tight junction integrity and promotes mucus production in tracheal epithelial cells To verify that IL-24 disrupts airway epithelial cell structure during asthma pathogenesis, we collected lung and tracheal tissues from mice and performed IF staining for the tight junction marker zonula occludens-1 (ZO-1) using an anti-ZO-1 antibody

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