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Previous reports suggest that B 12 deficiency-associated dysfunction of methionine synthase reduces the availability of methionine and subsequently S-adenosyl-methionine, which is an important methyl group donor (Figure 2)

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PLoS ONE 12(11):e0188334 Richardson MC, Guo M, Fauser BC, Macklon NS (2014) Environmental and developmental origins of ovarian reserve

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An excess mass of 79.9663 compared to the unmodified sequence, corresponding to the phosphate group, is observed in the precursor and in sequence ions than contain serine 39, serine 168 and serine 394