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In dried whole blood spots, we measured phosphatidylethanol (sensitivity, 8 ng/dl

The PCR was performed on genomic DNA isolated from individual colonies with the primers flanking the GPX2 gene sequence ( GPX2 _F: 5- TGATGGGGCAAGTAGGAGTC-3, GPX2 _R: 5- CCACACCTGCCCTTTATTGG-3)