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Similar to the hot plate test, animals are placed on the pre-cooled plate at a specific temperature (usually 410C) for a maximal observation period of 2030 s to minimize the risk of tissue damage (Marcotti et al., 2023

This work is supported by the Special Fund for Basic Research on Scientific Instruments of the National Natural Science Foundation of China (No.82160584)

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Three long unstructured regions in ZSWIM8 were not modelled owing to the absence of any interpretable density

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In contrast, kefir-fed mice maintained stable exploration of the central area across all trials and spent nearly five times more time (p = 0.03) in the inner zone than controls during the third test (Figure 3B), suggesting reduced anxiety