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Proc Natl Acad Sci U S A 119(8):e2107266119

NAC provides cysteine, a precursor to glutathione that is involved in metabolism of the toxic N-acetyl-p-benzoquinone imine (NAPQI) molecule

Curcumin alters EpRE and AP-1 binding complexes and elevates glutamate-cysteine ligase gene expression

Determination of MMP To determine MMP, cells were incubated with TMRM (50 nM

While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain

Don't mix in a blender or in hot liquids as it can destroy the liposomes