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Primary antibodies targeting TfR1, Fpn, FTH/L, GPX4, SLC7A11 and HO-1 were acquired from Abcam, while -actin was procured from Santa Cruz Biotechnology

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This indicates that Lira can improve liver fatty acid oxidation in obese mice, reduce liver fat accumulation, and promote hepatic lipolysis by activating the liver SIRT1/PGC-1/PEPCK pathway [67]