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Separation was achieved using a gradient of Solvent B (10 mM TEABC, pH 8.0, 90% ACN) and Solvent A (10 mM TEABC, pH 8.0) at the flow rate of 250 L/min

Severe adverse events have not been a pattern in community usage, but human safety data is limited to a few pilot studies

Contraindications and precautions Consider avoiding DSIP or consulting a physician if: Pregnant or breastfeeding (insufficient safety data) Taking naloxone or other opioid antagonists (may block effects) Using medications metabolized by peptidases (potential interactions) History of immune disorders (theoretical immunogenicity concern) Already using multiple sleep medications Drug interactions Because DSIP degrades via amino-peptidases in blood, medications affecting these enzymes (like the ACE inhibitor captopril) could theoretically alter DSIP metabolism

However, much of the available evidence comes from laboratory and animal studies, and human clinical research remains limited

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Side effects that are rare, delayed, or cumulative may not yet be identified