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After washing, cells in each well were treated with 25 l of staining solution containing Syto14 Green nucleic acid stain (1:2000 dilution, S7576 Thermo Scientific), Wheat Germ Agglutinin_Alexa Fluor 555 Conjugate (1:200 dilution, W32464 Thermo Scientific) and Hoechst 33342 (1:4000 dilution, 62249 Thermo Scientific) for 45 minutes at room temperature

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Monitoring immediate response: Watch for injection site reactions (normal mild redness or slight swelling resolves within hours)

Reason: Vitamin C helps boost glutathione activity and improves its absorption

Article Google Scholar Mullachery, P

They are also assessed for result delivery