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One week post-injection, mice were placed on a MASH-inducing diet enriched in fat, cholesterol and fructose (TD160785, Envigo) for 13 weeks

A B12 shot is an intramuscular injection of vitamin B12 , a crucial nutrient that helps with: Red blood cell production Nervous system function DNA synthesis Metabolism of carbohydrates, fats, and proteins Vitamin B12 is found in animal-based foods, so deficiencies are especially common in vegetarians, vegans, and individuals with absorption issues

Usually, the cell can remove reactive oxygen species (ROS) and reactive nitrogen species (RNS) through antioxidant molecules such as reduced glutathione (GSH) and primary defense enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione-dependent peroxidases (GPx) [1]

In addition, SOD1, C9ORF72, TDP-43 and FUS are also crucial molecular players associated with mitochondrial defects in ALS

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Horenberg AL, Houghton AM, Pandey S, Seshadri V, Guilford WH