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After 24 hours, cells were washed with saline to remove non-internalized bacteria and lysed with 0.1% saponin (Sigma-Aldrich) per 10-20 minutes at 37 C

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Amino acid losses during hemodialysis with polyacrylonitrile membranes: effect of intradialytic amino acid supplementation on plasma amino acid concentrations and nutritional variables in nondiabetic patients

doi:10.1111/j.1753-4887.2010.00273.x Visioli F, Davalos A

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Glucaric acid and ribonic acid had the most significant positive correlation with each other, and there were also highly positive correlations between L-acetylcarnitine and L-carnitine, and between taurine and glutathione (Table 3)