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Surgical model and experimental groups Animal studies were performed in accordance with the guidelines established by the Public Health Service Policy on Humane Care and Use of Laboratory Animals, federal and state regulations, and approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Pittsburgh

RNA and protein were removed by incubation first with 10 g DNase-free RNase-A at 37 C for 1 h, and then with 20 g proteinase K (Sigma) at 50 C for 4 h

Zhang LX, Lai JH, Liang ZS, Ashraf M

Acrodermatitis enteropathica: clinical manifestations and pediatric diagnosis

It contains a mixture of metals such as silver, copper and tin, in addition to mercury, which binds these components into a hard, stable and safe substance