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The HG + si-Nrf2 + DAPA group (si-Nrf2 + DAPA group) was cultured in medium containing 25 mmol/l glucose for 48 h, with the addition of 5 l of si-Nrf2 at a concentration of 20 M for 6 h, followed by culture in medium containing DAPA at a final concentration of 25 mol/l for 48 h

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After washing with PBST 0,1%, the membrane was incubated for 1h at room temperature (RT) with either Amersham ECL anti-mouse antibody (1:2000