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Then, the proteins were examined by mouse anti-GAS7 (Origene, 2F6, TA501756), anti-actin (Merck Millipore, MAB1501), anti-GFP (MBL, 598) and anti-glyceraldehyde-3-phosphate dehydrogenase (Santa Cruz Biotechnology, sc-166574) antibodies as primary antibody at 1:1000 dilution, followed by the secondary antibody of anti-mouse or rabbit IgG alkaline phosphatase conjugate (Promega) in PBS-T with 1:10000 dilution

The same redox flexibility of iron that can amplify oxidative stress is also what makes it indispensable for mitochondrial energy metabolism, which we have recently shown to be a limiting factor for glutathione synthesis required to protect against ferroptosis [29]

3C, 5D and EV4B)

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Slides were then developed with DAB for 5 min, rinsed with TBS, counterstained with hematoxylin, dehydrated, cleared, and mounted with a synthetic mounting medium

[PubMed Central ID: PMC7104012]