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doi: 10.1016/j.jad.2019.02.063 101 IzziBTirozziACerlettiCDonatiMBde GaetanoGHoylaertsMFet al

The cells were then treated according to the experimental setup for monocultures described in Section "Cell cultures and treatments"

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48 and 96 hours after treatment, cells were fixed with 4% paraformaldehyde, stained with DAPI (Invitrogen), and then mounted in mounting solution (20 mM n-propylgallate, 20% PBS, and 80% glycerol)

This review will provide recent and advanced findings focusing on how alcohol alters the aging process and aggravates Alzheimers disease with a focus on mitochondrial function