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Chromatographic separation was performed on a Waters XBridge HILIC column (100 mm 4.6 mm, 3.5 m) with a mobile phase consisting of (A) water with 0.2% formic acid and (B) acetonitrile with 0.1% formic acid

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This study revealed that PDPN binds to galectin 8 (GAL8), a secreted lectin produced by LECs, in a glycosylation-dependent manner, and this interaction promotes the activation of the pro-migratory integrin -1, facilitating the attachment of TAMs to LECs ( Figure 3A )