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On the subsequent day, the wells were blocked (200 L/well) with PBS/M (pH 7.4) for 1 h at room temperature and washed (250 L/well) four times with PBS/T (pH 7.4)

Laboratory studies demonstrate that FOXO4-DRI can eliminate chemotherapy-induced senescent cells that may contribute to administration resistance, while the timing of senolytic administration relative to chemotherapy requires careful optimization in experimental designs

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Studies have also shown stress-protective properties, with DSIP reducing stress-induced cortisol elevation and normalizing disrupted sleep patterns in stressed individuals

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