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Am J Cardiol 2019;124:1827-32

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When desalted APEX2 was again incubated with Amplex Red (100 M) and H 2 O 2 (100 M) its activity was unaffected by prior exposure to Na 2 S 4

The driving force of phase separation In physics, phase separation occurs when a molecule reaches its upper limit of dissolution or when the entropy of a solution is maximally maintained, pushing the molecules out of solution 31,33

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Background: SPG35 is caused by biallelic pathogenic variants in the FA2H , which encodes fatty acid 2hydroxylase (FA2H), an enzyme that functions in hydroxylation of free fatty acids