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RESULTS Developmental stage and sex modulate the cellular transcriptional state in the developing lung To gain a comprehensive understanding of the sex specific transcriptional changes in the neonatal lung at PND 1 (saccular stage of lung development), PND 7 (early alveolar stage of lung development) and PND 21 (late alveolar stage of lung development), we collected lungs from male and female neonatal mice under room air conditions and processed them for single cell RNA sequencing ( Figure 1A )