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Protoplasts were collected at the interphase and transferred to a filter-sterilized W5 solution (2 mM MES pH 5.8, 154 mM NaCl, 125 mM CaCl 2 .2H 2 O, 5 mM KCl, 5 mM glucose) prior to counting in a Rosenthal chamber

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Tesamorelin works through growth hormone pathways to target visceral fat specifically, while retatrutide activates GLP-1, GIP, and glucagon receptors to reduce overall body weight and liver fat

In brief, plated cells were incubated for 30 min at 37 C, 5% CO 2 in the staining solution (HBSS containing 1 g ml 1 Hoechst 33342 (62247, Invitrogen, Thermo Fisher Scientific) and either 5 M CellROXTM, 1 M FerroOrange or 1 M BODIPY 581/591 C11)