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Co-cultured cells were collected in one 15 ml falcon tube and lysed by RIPA buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) containing protease inhibitor cocktail (Sigma-Aldrich) and PMSF (Sigma-Aldrich) with 1 M Sodium-Orthovanadate pH 10(Sigma-Aldrich)

Adherence to the DASH and Mediterranean diets is associated with decreased risk for gestational diabetes mellitus

Ramesh A, Archibong AE, Huderson AC, Diggs DL, Myers JN, Hood DB, et al

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Pereira AAL, Lam M, Kanikarla Marie P, Raghav KPS, Morris VK, Brown H, Windham J, Duose DY, Overman MJ, Vilar Sanchez E, Wistuba II, Kipp P, Janku F, Sinha S, Kopetz S

Santambrogio), goat anti-rabbit FPN (1:1,000