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594 goat anti-mouse IgG1, Alexa Fluor, Invitrogen, United States) diluted in PBS containing 1% NGS were applied

Step 1: Determine Your Vial Concentration After reconstitution, your concentration depends on how much bacteriostatic water you add: Step 2: Calculate Units on Your Syringe Using a standard U-100 insulin syringe (100 units = 1 ml): Formula: Units to draw = (Desired dose in mcg Concentration in mcg/ml) 100 Example calculations for a 2 mg/ml (2000 mcg/ml) concentration: Example calculations for a 3 mg/ml (3000 mcg/ml) concentration: Quick Reference Card For a 9 mg vial reconstituted with 3 ml bacteriostatic water (3 mg/ml): Reconstitution: Getting It Right Proper mixing ensures your sermorelin maintains its potency and delivers accurate doses