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However, their precise temporal sequence requires further investigation

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(Reference ODoherty, Freedman and Hollenbeck62) These findings highlight the impact of not only excess weight, as assessed through BMI, but also body fat distribution on GERD (Reference Krner, Corts and Lukens59)

After washing with PBS for 3 times, cells were permeabilized with 0.1% Triton X-100 for 10 min and blocked with 5% normal goat serum with 0.01% Triton X-100 in PBS for 1 hr at room temperature

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