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Cells were lysed by vigorous vortexing, incubated for 10 min at 4 C, and centrifuged at 14,000 rpm for 10 min at 4 C

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Furthermore, UPF contain reduced levels of fiber content, which may facilitate increased rates of eating as well as an increased consumption of UPF, which in some cases leads to a significant reduction in the levels of satiety (122)

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