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Description

3T3-L1 cells were cultured in 60 mm diameter dishes (8.4 104 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 M dexamethasone, 10 g/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)

Unlike peptide-based incretins, this is a Small Molecule Inhibitor targeting the cytosolic enzyme Nicotinamide N-methyltransferase (NNMT)

both were shifted by over 0.1 ppm compared with the -H in the monomers of tartaric acid and l-carnitine, which supports that proton transfer occurred between tartaric acid and l-carnitine to form an IL (Fig