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Thus, plants need to be grown in the presence of high selenium levels to accumulate meaningful amounts of methylselenocysteine

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The cells were harvested by centrifugation (1000 x g , 2 min) and resuspended in lysis buffer (5% w/v 5-sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA (KPE buffer [35])) together with 425600 m-diameter acid-washed glass beads (Sigma) at a buffer/bead ratio of 1:2 by volume

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The results showed no histological alterations, indicative of the high biocompatibility and biosafety

54 These species-specific patterns reflect underlying physiological characteristics, including the cell envelope, efflux systems, and thiol metabolism