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Folate as a natural antioxidant vitamin occurs naturally in orange juice, strawberries, dark green leafy vegetables and peanuts [2]

To experimentally assess the functional consequences of our nrf2a5 mutated allele, we tested its ability to activate the transcription of an ARE-driven firefly luciferase reporter gene following a previously published protocol (Mukaigasa et al, 2012)

Furthermore, the original co-crystallized ligand inside the active site may affect the conformation of the protein and, as a consequence, the binding mode of other ligands

doi: 10.1128/aac.48.11.4414-4421.2004 141 BuchheitKMShawDChuppGLehtimakiLHefflerEFinney-HaywardTet al

The clinical evidence for the diverse strategies discussed above, ranging from direct antioxidants to multi-targeted agents, is summarized in Table 1, which illustrates the varied outcomes and highlights the ongoing translational challenges