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The alternative strategy focuses on monitoring epigenetic changes during treatment and intervening with epigenetic drugs before the stabilization of resistant epigenetic states, which may delay or prevent resistance developmentpotentially enabling cancer to be managed as a chronic condition [181]

Chapter Six - Mitochondria and Reactive Oxygen Species in Aging and Age-Related Diseases

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doi: 10.1111/j.1365-2133.2009.09198.x 37

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Beads were then washed three times with binding buffer, and bound complexes were eluted in 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 250 mM imidazole elution buffer