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The drug-induced hypersensitization of the brain reward circuits is hypothesized to cause a pathological transition from drug liking to wanting that underlies compulsive substance use as previously demonstrated with chronic challenges of morphine, cocaine, ethanol, nicotine, and cannabinoids ( All mice were taken out from the IntelliCage once daily (in the morning) to receive their assigned 28-day drug sensitization regimen

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Subsequently, the samples were permeabilized with 0.3% Triton X-100 in PBS at room temperature for 10 min

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