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Each of the 430 compounds from a SelleckChem Kinase Inhibitor Library (subset of library #L1200) were combined at 100 M in wells of a polypropylene 96-well Tube Plates (Agilent) with TcPINK1 D337N at 0.5 mg/mL (10 M) and SYPRO Orange Protein Gel Stain 5000X (Thermo Fisher Scientific) at 6X in a 300 mM NaCl, 20 mM TrisHCl pH 8, 5 mM DTT, 5% DMSO buffer

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Additionally, inhibition of NNMT using MNAM resulted in a decline in performance during anaerobic endurance exercise in rats (Zhou et al., 2018)

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Notice side effects such as swelling, severe headaches, chest pain, or vision changes

Spermidine also protects DNA and mitochondria, acts as a signalling molecule and has countless other benefits