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LC-MS/MS Experiments were performed on an Orbitrap Fusion Lumos (Thermo) that was coupled to a Vanquish Neo ultra-high-performance liquid chromatography (UHPLC) system (Thermo)

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GST-GFP was readily observed (Figure 1C, lower panel) and purified with a yield of approximately 300 mg/L of culture (data not shown)

In lung cancer, the TME favors M2 polarization, characterized by enhanced OXPHOS, FAO, and upregulation of ARG1 and IL-10

For instance, continuous increases in myelin debris cannot be accommodated by microglia in the long-term, which subsequently leads to dysfunctional lysosomal activity and a noticeable increase in insoluble lipofuscin-like granulesa marker of aging, dystrophy and possibly senescencein mice (Safaiyan et al., 2016)