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All mycelia were loaded with 5 M Fluo-3-AM ester, a membrane-permeable calcium indicator, for 40 min at 37C

However, under nutrient-limited conditions, bacteria can use as fermentable substrate the EPS previously produced (Rios-Covian et al., 2016)

on alternate days until there is no further improvement

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[3] One such signaling pathway that Mod GRF (1-29) is believed to activate involves adenylyl cyclase