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The membranes were incubated with GAPDH monoclonal antibody (1:5000, Cat# 81115-1-RR, SAB, China), -SMA monoclonal antibody (1:1000, Cat# 19245T, CST, USA), collagen I polyclonal antibody (1:1000, Cat# 14695-1-AP, Proteintech, China), p-AMPK monoclonal antibody (1:1000, Cat# 50081 S, CST, USA), AMPK monoclonal antibody (1:1000, Cat# 5832T, CST, USA), HO-1 monoclonal antibody (1:2000, Cat# ab189491, Abcam, UK), NQO-1 monoclonal antibody(1:10000, Cat# ab80588, Abcam, UK) and Nrf2 monoclonal antibody (1:2000, Cat# 16396-1-AP, Proteintech, China) at 4 overnight

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