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Petznek H, Kleiter M, Tichy A, Fuchs-Baumgartinger A, Hohenadl C (2014): Murine xenograft model demonstrates significant radio-sensitising effect of liposomal doxorubicin in a combination therapy for Feline Injection Site Sarcoma

You dont want to cover the needs of only half the population though, so you add two relative standard deviations to cover more like 98% of the population, which just means you up the average requirement by 20 or 40%

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Serum-free or low-serum conditions are essential because serum IGFBPs in the media would partially sequester even LR3 at the highest serum concentrations