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In turn, these alterations observed in the different organs and systems have a direct impact on the PNIE, as they influence in the immune responses (i.e., liver inflammation after SCI impairs neurological recovery after SCI), endocrine dysregulation (i.e., through the release of cardiomyokines by the heart) and in the nervous system/psyche (i.e., through the heart-brain, lung-brain or kidney-brain axis) [102, 251,252,253,254,255]

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High content image analysis pipelines were applied to quantify cellular responses as described previously in detail (Wink et al

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11 Some of these targets are shown in Table 1