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Resuspend the cell pellet in Solution B (for the four-step gradient) OR the 21.6% (w/v) iodixanol solution (for the two-step gradient)

Heme produces signaling molecules, such as CO, biliverdin (BV), BR and Fe 2+ , through the catalysis of HO-1

PDT has been studied since the 1980s (268, 269), and has shown promising efficacy in many glioma preclinical studies (270272)

These observations demonstrate that individual proteins can undergo simultaneous lactylation and acetylation modifications, potentially generating synergistic effects

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Without its signal sequence, AP accumulates in the cytoplasm, and only a tiny fraction is exported to the periplasm (Eben and Imlay, 2023)