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The following day, Ni-NTA resin equilibrated in buffer A was added over the cleaved laccase samples and incubated at 25 C and 1,000 rpm in a thermomixer comfort shaker (Eppendorf) for 4 h

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Risk factors for deficiency include long-term antacid medications use, metformin therapy, GI disorders, surgery, and vegetarian/vegan diets

Human muscle protein synthesis is modulated by extracellular, not intramuscular amino acid availability: a dose-response study

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They support healthy fat metabolism and liver detoxification, which can make weight management easier, but nothing is a magic bullet