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For the NADPH titration of SjTGR WT or SjTGR R317A , the NADPH concentration was 400 M, and protein concentration was 20 M

Fedorowski, A

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10.1016/j.numecd.2009.05.014 78 PervinZ.StephenJ

Fractions (~500 l each) were collected sequentially from the top using reverse mode on the Autodensiflow system and stored at 80 C until further analysis