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If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers

Despite certain discrepancies between the predicted and the actual results, the computation tools successfully identified nanobody mutants with significantly improved thermal stability, demonstrating the feasibility of using this prediction scheme for identifying stable nanobody mutants with pAcF incorporated

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Fisher exact test was used to assess classification of steatosis between VCTE and MRE