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This method could confirm the stability of the target protein in a complex with the candidate ligands and determine the stability and rigidity of proteinligand complexes in a specific artificial environment, such as the body [32]

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Western blotting Renal tissues and HK-2 cells were lysed using radioimmunoprecipitation assay buffer (Servicebio)

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Safety of high-dose nicotinamide: A review

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