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After incubation at 4 C for 16 h, the streptavidin resin was collected and washed with 1% SDS (2X), 4 M urea (2X), PBS (2X) and ddH 2 O (2X), resuspended in loading buffer, boiled to release the biotin-conjugated proteins, and analyzed by immunoblot

GABA receptors are probably the most common kind in the mammalian nervous system, and GABA is the primary inhibitory neurotransmitter in the brain (Tyagi et al ., 2007a, b)

Statistical differences between groups were identified by one-way analysis of variance (ANOVA) followed by the Duncan post hoc test

Together, these examples underscore the importance of studying metabolic remodeling during human neurodevelopment and emphasize the need for reliable, neuron-specific model systems that can recapitulate early stages of differentiation and bioenergetic specialization

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Use any of these options to make a tasty BCAA drink or smoothie alongside your favorite fruits, veggies and superfood mix-ins