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A helium bag was used between the sample, crystals and detector to minimize attenuation of the fluorescence from air and reduce background from diffusely scattered radiation

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Diabetes 55 , 341348 (2006)

Moreover, KO mice for liver IGF-1 gene developed muscle insulin resistance (it is important to mention that skeletal muscle myocytes express high number of IGF-1R), showing an increase in insulin concentrations and a substantial decrease in the insulin-induced autophosphorylation of the insulin receptor and IRS in skeletal muscle (being normal in liver and white adipose tissue)