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Ethanol-fixed cells were stained with propidium iodide (PI) at room temperature for 1 h in PBS containing 20 g/ml PI (Sigma-Aldrich, St

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Comparative AUC (n=819) for predicting LRE were 0.84 (ELF), 0.79 (FIB4), 0.74 (APRI), 0.67 (AST:ALT ratio) and for PHTN-LRE were 0.90 (ELF), 0.85 (FIB4), 0.81 (APRI), 0.75 (AST:ALT ratio)

Bio-Rad was used to transfect the brain slices with a human htt exon-1 expression construct containing a 73 CAG repeat (HttN90Q73) in the gWiz backbone (Genlantis) together with a YFP expression construct to visualize transfected neurons