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Moreover, it promotes mitochondrial damage via reactive oxygen species (ROS) accumulation [30] and synergizes with doxorubicin (DOX) to exert potent antitumor effects [31]

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The bound endogenous GS was eluted from cross-linked IgG using Elution buffer pH 2.8 as per the manufacturers protocol

PMID: 94 LiuTHanSYaoYZhangG

Quantification of metabolites by LC-MS/MS To quantify polar metabolites concentrations, we used TSQ Vantage LC-MS interfaced with Ultimate 3000 Liquid Chromatography system (Thermo Scientific) and Triple quadruple mass spectrometry to detect metabolites from tissues or cells