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Simultaneously, studies using molecular biology detection and pharmacological intervention confirmed that heat stress triggers cPLA2 activation and increases the level of its metabolite arachidonic acid to participate in lipid peroxidation in the mouse heatstroke model, further promoting the occurrence of ferroptosis, thereby aggravating the progression of ALI after heat stress [48]

Protein carbonyl assay RWPE-1, LNCaP, COS-7, and COS-7-OPH cells were grown in 25 cm 3 cell culture flasks to 80% confluence

doi: 10.1172/JCI143774 238 SencioVBarthelemyATavaresLPMaChadoMGSoulardDCuinatCet al

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Morgan BP, Chamberlain-Banoub J, Neal JW, Song W, Mizuno M, Harris CL

Culture-Independent Approach Besides that, different approaches have been developed to study the microbiome, including clone libraries, terminal restriction fragment length polymorphism (T-RFLP), quantitative PCR (qPCR), transcriptome microarrays, as well as high throughput sequencing technologies (Illumina sequencing and 454 pyrosequencing)