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Resuspended samples were then reacted with 20 mM TCEP for 3 min and then mixed with 40 mM IAM and further incubated at RT in the dark for 30 min

For example, whether mesangial cell ferroptosis contributes to glomerular sclerosis or if endothelial ferroptosis exacerbates renal microvascular dysfunction in DN has not been rigorously characterized