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The recombinant proteins were then eluted with lysis buffer containing 150 mM imidazole

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MTHFR Gene Variants with Hyperhomocysteinemia and Cardiovascular Diseases Elevated Hcy levels are considered an independent risk factor for the development of CVD, which encompasses CAD, MI, IS, hypertension, and thrombosis [84,85]

Vegetarians and vegans are often low in vitamin B12, which may cause an increase in homocysteine levels (19,20)

In bacteria, tRNA Tyr identity elements include a G1-C72 base pair, which are inverted in archaea and eukaryotes (C1-G72) (Bedouelle 1990